Production of Poly (3-hydroxybutyrate-co-3- Hydroxyvalerate) by a Novel Bacillus Ou40 T from Inexpensive Carbon Sources

The goals of this work are to characterize and evaluate the ability of strain OU40 to produce poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) using various agricultural wastes, isolated from polluted water.Growth of the isolate OU40 was assessed in mineral media containing glucose, starch, bagasse, whey and rice bran without adding any precursors. Biopolymers produced by strain OU40 had an average molecular weight 1,304 kDa to 2,332 kDa with the melting point 155-160 Tm( C) and percentage of crystallinity 4050%. Based on phenotypical, biochemical and genotypic investigations, strain OU40 is assigned to the genus Bacillus. The DNA-DNA relatedness between OU40 and Bacillus cereus ATCC14579 (AE016877) was found 60% of mean similarity, 4.24 SD value, ∆Tm value of 6% and G+C content was 46.5%.These result suggests that the strain could be a novel genomic species.The 16S rRNA gene sequence is deposited in EMBL/Genbank with accession number FN663629.The type strain is OU40 (=JCM17287=CCM7835 =DSM24141).